產(chǎn)品詳情
簡(jiǎn)單介紹：
CCL-138 Detroit 562 人咽頭癌胸水轉(zhuǎn)移細(xì)胞 ,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件,
詳情介紹：
CCL-138 Detroit 562 人咽頭癌胸水轉(zhuǎn)移細(xì)胞
Karyotype
Modal number = 64; range = 58 to128
A large subterminal marker chromosome, arm ratio 3:4, is found in 94% of the cells karyotyped. Five to 6 minute chromosomes are present in each cell. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines
Clinical Data
Genes Expressed
Virus Susceptibility
Human poliovirus 1
Vesicular stomatitis virus CCL-138 Detroit 562 人咽頭癌胸水轉(zhuǎn)移細(xì)胞
Comments
| Organism | Homo sapiens, human |
|---|---|
| Tissue | pharynx: derived from metastatic site: pleural effusion |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level | 1 |
| Disease | pharyngeal carcinoma |
| Age | ***** |
| Gender | female |
| Ethnicity | Caucasian |
| Storage Conditions | liquid nitrogen vapor phase |
A large subterminal marker chromosome, arm ratio 3:4, is found in 94% of the cells karyotyped. Five to 6 minute chromosomes are present in each cell. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines
*****
Caucasian
female
keratin
Vesicular stomatitis virus CCL-138 Detroit 562 人咽頭癌胸水轉(zhuǎn)移細(xì)胞
The cells are positive for keratin by immunoperoxidase staining.
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
|---|---|
| Subculturing |
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
|
| Cryopreservation |
Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage Temperature: liquid nitrogen vapor phase
|
| Culture Conditions |
Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C CCL-138 Detroit 562 人咽頭癌胸水轉(zhuǎn)移細(xì)胞
|
