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CRL-2586 EOMA 小鼠血管內(nèi)皮瘤細胞，ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和培養(yǎng)條件,
詳情介紹：
CRL-2586 EOMA 小鼠血管內(nèi)皮瘤細胞
ATCC® Number: CRL-2586™ Price: $329.00
Designations: EOMA
Depositors: R Auerbach
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus (mouse)
Morphology: endothelial
Source: Disease: hemangioendothelioma
Strain: 129
Cell Type: endothelial
Cellular Products: angiotensin converting enzyme (ACE) [51514]
thrombospondin [51514]
cathepsin L [47148]
endostatin [47148]
interleukin-6 (interleukin 6, IL-6) [53484]
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1980
Receptors: acetylated low density liproprotein [51514]
Tumorigenic: Yes
Antigen Expression: CD31 +
vascular addressin +
CD45 (Ly5-T200) +
Age: *****
Comments: The EOMA cell line was originally derived in 1980 from a mixed hemangioendothelioma arising in an ***** mouse. [51514]
The cells synthesize angiotensin-converting enzyme, express surface receptors for acetylated low density lipoprotein, produce thrombospondin and show intracellular staining with an antibody to von Willebrand factor. [51514]
Cathepsin L is secreted by EOMA cells and is responsible for the generation of endostatin L. [47148]
Although constitutive cytokine gene expression exists in EOMA cells, the level of IL-6 mRNA is prominently elevated by incubation with Liposome encapsulated hemoglobin (LEH). [53484]
The cells constitutively express the vascular addressin identified by antibody MECA-99.
EOMA cells exhibit characteristic endothelial cell properties, such as rearrangement into tubelike structures on Matrigel and retention of cobblestone morphology at confluence. They behave in vitro in a manner similar to microvascular endothelial cells. [51514]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol:
1.Remove and discard culture medium.
2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
| ATCC® Number: | CRL-2586™ | Price: | $329.00 | |||||||||||||||||||||||||||||||||||||||||||||||||
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Designations: EOMA
Depositors: R Auerbach
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus (mouse)
Morphology: endothelial
Source: Disease: hemangioendothelioma
Strain: 129
Cell Type: endothelial
Cellular Products: angiotensin converting enzyme (ACE) [51514]
thrombospondin [51514]
cathepsin L [47148]
endostatin [47148]
interleukin-6 (interleukin 6, IL-6) [53484]
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1980
Receptors: acetylated low density liproprotein [51514]
Tumorigenic: Yes
Antigen Expression: CD31 +
vascular addressin +
CD45 (Ly5-T200) +
Age: *****
Comments: The EOMA cell line was originally derived in 1980 from a mixed hemangioendothelioma arising in an ***** mouse. [51514]
The cells synthesize angiotensin-converting enzyme, express surface receptors for acetylated low density lipoprotein, produce thrombospondin and show intracellular staining with an antibody to von Willebrand factor. [51514]
Cathepsin L is secreted by EOMA cells and is responsible for the generation of endostatin L. [47148]
Although constitutive cytokine gene expression exists in EOMA cells, the level of IL-6 mRNA is prominently elevated by incubation with Liposome encapsulated hemoglobin (LEH). [53484]
The cells constitutively express the vascular addressin identified by antibody MECA-99.
EOMA cells exhibit characteristic endothelial cell properties, such as rearrangement into tubelike structures on Matrigel and retention of cobblestone morphology at confluence. They behave in vitro in a manner similar to microvascular endothelial cells. [51514]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol:
1.Remove and discard culture medium.
2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
