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MDA-MB-436
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R Cailleau
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Biosafety Level:
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1
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frozen
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See Propagation
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adherent
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Homo sapiens (human)
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pleomorphic with multinucleated component cells
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Organ: mammary gland; breast
Disease: adenocarcinoma
Derived from metastatic site: pleural effusion
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tubulin; actin
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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No
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Amelogenin: X
CSF1PO: 12
D13S317: 10
D16S539: 9,11
D5S818: 13
D7S820: 10
THO1: 9.3
TPOX: 8
vWA: 14,20
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modal number = 45
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AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 1
PGM1, 1
PGM3, 1
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43 years
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female
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Caucasian
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ATCC complete growth medium: Leibovitz's L-15 medium with 10 mcg/ml insulin, 16 mcg/ml glutathione, 90%; fetal bovine serum, 10%
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Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 2 to 3 times per week
Subcultures are prepared by scraping. Remove spent medium, add 3 to 5 ml of fresh medium, dislodge cells from the floor of the flask, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
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Culture medium, 95%; DMSO, 5%
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1206: Brinkley BR, et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337
22429: Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779
22656: Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202
23079: Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903
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