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ACHN
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TF Hogan
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Biosafety Level:
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1
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frozen
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See Propagation
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adherent
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Homo sapiens (human)
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epithelial
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Organ: kidney
Disease: renal cell adenocarcinoma
Derived from metastatic site: pleural effusion
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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Isolation date: November, 1979
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Yes
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Amelogenin: X
CSF1PO: 11
D13S317: 12
D16S539: 12,13
D5S818: 12
D7S820: 9,11
THO1: 8
TPOX: 8,11
vWA: 16,17
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G6PD, B
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22 years
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male
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Caucasian
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ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10.
Atmosphere: air, 95; carbon dioxide (CO2), 5
Temperature: 37.0°C
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Protocol:
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Remove and discard culture medium.
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Briefly rinse the cell layer with 0.25 (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
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Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
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Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
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Add appropriate aliquots of the cell suspension to new culture vessels.
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Incubate cultures at 37C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
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Freeze medium: Complete growth medium, 95; DMSO, 5
Storage temperature: liquid nitrogen vapor temperature
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Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
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91982: Kochevar J. Blockage of autonomous growth of ACHN cells by anti-renal cell carcinoma monoclonal antibody 5F4. Cancer Res. 20: 2968-2972, 1990. PubMed: 2334900
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