<cite id="pwzsq"><rp id="pwzsq"></rp></cite>


      
欧美视频精品免费覌看,7777久久亚洲中文字幕蜜桃,免费观看的av在线播放,国产一区二区三区免费主播,久久精品aⅴ无码中文字幕,中文字幕日韩有码国产,啊灬啊灬啊灬快灬深学长慢视频,国产片av片永久免费观看 

    
      
        
      
            
      
                
      
                    
                
      
                
                
      
                    
      
                        
      
                            
      
                                
      
                                    
                                
      
                                
      
                                    
                                    
      
                                        
      
                                            
      
                                                
      
        
      
        
      
            
      
                
      
                    
                    
                    
                    
                    
                
      
                
      
                
      
                    
      
                        
      
                            產品詳情
                        
      
                        
      
                            
      
                                
                                
                                
                                
                            
      
                            
      
    
      
    
      
        
      
    
      
            
      

                
      
                    
                    
        
                          
      • 產品名稱：CRL-2190 HK-2 人腎近曲小管上皮細胞
        • 
                          
      • 產品型號：CRL-2190
        • 
                          
      • 產品廠商：美國標準生物品收藏中心（ATCC）
        • 
                          
      • 產品價格：0
        • 
                          
      • 折扣價格：0
        • 
                          
      • 產品文檔：
                          
        • 




                    
      
                    
        
                           
      • 
                             
        
                               
                             
        
                         
        • 
                    
      
                     
      
                    你添加了1件商品 查看購物車
                    
      

                
      
            
      
    
      

    
      
        
      
            簡單介紹：
        
      
        
      
            CRL-2190 HK-2 人腎近曲小管上皮細胞，原代細胞|細胞系|細胞株|菌種；細胞庫管理規范，提供的細胞株背景清楚，提供參考文獻和培養條件！
        
      
    
      

    
      
        
      
            詳情介紹：
        
      
        
      
            CRL-2190 HK-2 人腎近曲小管上皮細胞
      

      
    
        
      
            ATCC® Number: 
            CRL-2190™    
            Price: 
            $272.00
        
      
        
      
            
            
      
                
                    
      
                        Designations: 
                        HK-2
                    
      
                    
      
                        Depositors: 
                         RA Zager 
                    
      
                    
      
                        Biosafety Level:
                        2 [Cells Contain Papilloma viral DNA sequences ]
                    
      
                    
      
                        Shipped: 
                        frozen 
                    
      
                    
      
                        Medium & Serum: 
                        See Propagation
                    
      
                    
      
                        Growth Properties:
                        adherent
                    
      
                    
      
                        Organism:
                        Homo sapiens (human)
                    
      
                    
      
                        Morphology:
                        epithelial
      
                        
      
                              		
                    
      
                    
      
                        Source:
                        Organ: kidney, cortex 
      
                        Tissue: proximal tubule 
      
                        Cell Type: human papillomavirus 16 (HPV-16) transformed      		
                    
      
                    
      
                        Cellular Products:
                        alkaline phosphatase; gamma glutamyltranspeptidase; leucine aminopeptidase; acid phosphatase; cytokeratin; alpha 3, beta 1 integrin; fibronectin
                    
      
                    
      
                        Permits/Forms:
                        In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
                    
      
                    
      
                         
                    
      
                    
      
                        Receptors:
                        epidermal growth factor (EGF), expressed
                    
      
                    
      
                        DNA Profile (STR):
                        Amelogenin: X,Y 
      
                        CSF1PO: 13 
      
                        D13S317: 9 
      
                        D16S539: 11,12 
      
                        D5S818: 12 
      
                        D7S820: 10,11 
      
                        THO1: 9 
      
                        TPOX: 8,9 
      
                        vWA: 17,18      		
                    
      
                    
      
                        Age: 
                        ***** 
                    
      
                    
      
                        Gender: 
                        male 
                    
      
                    
      
                        Comments:
                        HK-2 (human kidney 2) is a proximal tubular cell (PTC) line derived from normal kidney. 
      
                        The cells were immortalized by transduction with human papilloma virus 16 (HPV-16) E6/E7 genes. 
      
                        The recombinant retrovirus vector pLXSN 16 E6/E7 containing the HPV-16 E6/E7 genes was used to transfect the ectotropic packaging cell line Psi-2. 
      
                        Virus produced by the Psi-2 cells was used to infect the amphotropic packaging cell line PA317 (see ATCC CRL-9078). 
      
                        Virus produced by the PA317 cells was used to transduce primary PTCs. 
      
                        Although pLXSN 16 E6/E7 also confers resistance to neomycin, selection in G418 was not used to isolate transduced clones. 
      
                        The cell line appears to be derived from a single cell based on Southern and FISH analysis. 
      
                        The E6/E7 genes are present in the HK-2 genome as determined by PCR. 
      
                        The cells retain a phenotype indicative of well differentiated PTCs. 
      
                        They are positive for alkaline phosphatase, gamma glutamyltranspeptidase, leucine aminopeptidase, acid phosphatase, cytokeratin, alpha 3,beta 1 integrin, and fibronectin. 
      
                        The cells are negative for factor VIII related antigen, 6.19 antigen and CALLA endopeptidase. 
      
                        HK-2 cells retain functional characteristics of proximal tubular epithelium such as Na+ dependent / phlorizin sensitive sugar transport and adenylate cyclase responsiveness to parathyroid, but not to antidiuretic hormone. 
      
                        The cells are capable of gluconeogenesis as evidenced by their ability to make and store glycogen. 
      
                        HK-2 cells are anchorage dependent. 
      
                        The cells will not grow in methylcellulose, soft agar or suspension. 
      
                        HK-2 cells can reproduce experimental results obtained with freshly isolated PTCs.      		
                    
      
                    
      
                        Propagation: 
                        ATCC complete growth medium: The base medium for this cell line is provided by Invitrogen (GIBCO) as part of a kit: Keratinocyte Serum Free Medium (K-SFM), Kit Catalog Number 17005-042. This kit is supplied with each of the two additives required to grow this cell line (bovine pituitary extract (BPE) and human recombinant epidermal growth factor (EGF). To make the complete growth medium, you will need to add the following components to the base medium:
                        
        
                            
      • 0.05 mg/ml BPE - provided with the K-SFM kit
        • 
                            
      • 5 ng/ml EGF - provided with the K-SFM kit. NOTE: Do not filter complete medium. 
        
                            Atmosphere: air, 95%; carbon dioxide (CO2), 5% 
        
                            Temperature: 37.0°C 
        
                            Growth Conditions: Cell growth is dependent on epidermal growth factor. The cells should not be allowed to become confluent. Subculture at 80% of confluence.
        • 
                        
      
                              		
                    
      
                    
      
                        Subculturing: 
                        Protocol: 
                        
        
                            
      1. Remove and discard culture medium.
        2. 
                            
      2. Briefly rinse the cell layer with 0.05% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
        3. 
                            
      3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
        
                            Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
        4. 
                            
      4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
        5. 
                            
      5. To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximately 125 xg for 5 to 10 minutes.Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.
        6. 
                            
      6. Incubate cultures at 37°C.
        7. 
                        
      
                              		
                    
      
                      
            
      
                  		
        
      
          

      
        
      
    
      

      
 
    
      

      

                            
      
    
      
    
      
        
      

  
      


          
      
              姓名：
              
      
                  
              
      
          
      
          
      
              電話：
              
      
                  
              
      
          
      
          
      
                  Email：
                  
      
                      
                  
      
              
      
          
      
              您的需求：
              
      
                  
              
      
          
      
          
      
               
              
      
                  
                  
              
      
          
      

  
      
  

      

    
      

      

                        
      
                    
      
                
      
            
      
        
      
    
      
                                            
      
                                        
      
                                    
      
                                
      
                            
      
                        
      
                    
      
                
      
                
            
      
		 		
      滬公網安備 31010902002429號
      
		 	
      
		 
      
        
      
    
      
 


主站蜘蛛池模板：
亚洲一区二区有码在线|
国产成人亚洲综合色婷婷秒播|
人妻av一区二区三区精品|
国产免费制服丝袜调教视频|
超碰狠狠干|
www.国产在线观看|
小sao货水好多真紧|
a国产一区二区免费入口|
亚洲va在线∨a天堂va欧美va|
爱操影院|
国产又色又爽又黄的网站免费|
四虎国产精品一区二区|
久操不卡|
欧洲中文字幕一区二区|
福利二区|
亚洲伊人色|
国产综合AV|
中国少妇人妻xxxxx|
中国孕妇变态孕交xxxx|
久热最新精品在线视频|
日韩精品国产二区三区|
日韩成人无码影院|
亚洲欧美?va天堂人熟伦|
91孕妇精品一区二区三区|
亚洲无码社区|
亚洲?日韩?中文?无码?制服|
人妻系列AV无码专区|
国模久久|
国产精品久久久久久人妻精品动漫|
亚洲成人精品|
中文字幕日产无码|
欧美亚洲|
日韩av裸体在线播放|
69国产精品久久久久久人|
超碰在线91|
国产乱妇无码大片在线观看|
革吉县|
把插八插露脸对白内射|
国产偷伦|
色综合久久成人综合网|
久久久久久亚洲AV成人无码激情|