<cite id="pwzsq"><rp id="pwzsq"></rp></cite>


      
欧美视频精品免费覌看,7777久久亚洲中文字幕蜜桃,免费观看的av在线播放,国产一区二区三区免费主播,久久精品aⅴ无码中文字幕,中文字幕日韩有码国产,啊灬啊灬啊灬快灬深学长慢视频,国产片av片永久免费观看 

    
      
        
      
            
      
                
      
                    
                
      
                
                
      
                    
      
                        
      
                            
      
                                
      
                                    
                                
      
                                
      
                                    
                                    
      
                                        
      
                                            
      
                                                
      
        
      
        
      
            
      
                
      
                    
                    
                    
                    
                    
                
      
                
      
                
      
                    
      
                        
      
                            產品詳情
                        
      
                        
      
                            
      
                                
                                
                                
                                
                            
      
                            
      
    
      
    
      
        
      
    
      
            
      

                
      
                    
                    
        
                          
      • 產品名稱：CRL-2190 HK-2 人腎近曲小管上皮細胞
        • 
                          
      • 產品型號：CRL-2190
        • 
                          
      • 產品廠商：美國標準生物品收藏中心（ATCC）
        • 
                          
      • 產品價格：0
        • 
                          
      • 折扣價格：0
        • 
                          
      • 產品文檔：
                          
        • 




                    
      
                    
        
                           
      • 
                             
        
                               
                             
        
                         
        • 
                    
      
                     
      
                    你添加了1件商品 查看購物車
                    
      

                
      
            
      
    
      

    
      
        
      
            簡單介紹：
        
      
        
      
            CRL-2190 HK-2 人腎近曲小管上皮細胞，原代細胞|細胞系|細胞株|菌種；細胞庫管理規范，提供的細胞株背景清楚，提供參考文獻和培養條件！
        
      
    
      

    
      
        
      
            詳情介紹：
        
      
        
      
            CRL-2190 HK-2 人腎近曲小管上皮細胞
      

      
    
        
      
            ATCC® Number: 
            CRL-2190™    
            Price: 
            $272.00
        
      
        
      
            
            
      
                
                    
      
                        Designations: 
                        HK-2
                    
      
                    
      
                        Depositors: 
                         RA Zager 
                    
      
                    
      
                        Biosafety Level:
                        2 [Cells Contain Papilloma viral DNA sequences ]
                    
      
                    
      
                        Shipped: 
                        frozen 
                    
      
                    
      
                        Medium & Serum: 
                        See Propagation
                    
      
                    
      
                        Growth Properties:
                        adherent
                    
      
                    
      
                        Organism:
                        Homo sapiens (human)
                    
      
                    
      
                        Morphology:
                        epithelial
      
                        
      
                              		
                    
      
                    
      
                        Source:
                        Organ: kidney, cortex 
      
                        Tissue: proximal tubule 
      
                        Cell Type: human papillomavirus 16 (HPV-16) transformed      		
                    
      
                    
      
                        Cellular Products:
                        alkaline phosphatase; gamma glutamyltranspeptidase; leucine aminopeptidase; acid phosphatase; cytokeratin; alpha 3, beta 1 integrin; fibronectin
                    
      
                    
      
                        Permits/Forms:
                        In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
                    
      
                    
      
                         
                    
      
                    
      
                        Receptors:
                        epidermal growth factor (EGF), expressed
                    
      
                    
      
                        DNA Profile (STR):
                        Amelogenin: X,Y 
      
                        CSF1PO: 13 
      
                        D13S317: 9 
      
                        D16S539: 11,12 
      
                        D5S818: 12 
      
                        D7S820: 10,11 
      
                        THO1: 9 
      
                        TPOX: 8,9 
      
                        vWA: 17,18      		
                    
      
                    
      
                        Age: 
                        ***** 
                    
      
                    
      
                        Gender: 
                        male 
                    
      
                    
      
                        Comments:
                        HK-2 (human kidney 2) is a proximal tubular cell (PTC) line derived from normal kidney. 
      
                        The cells were immortalized by transduction with human papilloma virus 16 (HPV-16) E6/E7 genes. 
      
                        The recombinant retrovirus vector pLXSN 16 E6/E7 containing the HPV-16 E6/E7 genes was used to transfect the ectotropic packaging cell line Psi-2. 
      
                        Virus produced by the Psi-2 cells was used to infect the amphotropic packaging cell line PA317 (see ATCC CRL-9078). 
      
                        Virus produced by the PA317 cells was used to transduce primary PTCs. 
      
                        Although pLXSN 16 E6/E7 also confers resistance to neomycin, selection in G418 was not used to isolate transduced clones. 
      
                        The cell line appears to be derived from a single cell based on Southern and FISH analysis. 
      
                        The E6/E7 genes are present in the HK-2 genome as determined by PCR. 
      
                        The cells retain a phenotype indicative of well differentiated PTCs. 
      
                        They are positive for alkaline phosphatase, gamma glutamyltranspeptidase, leucine aminopeptidase, acid phosphatase, cytokeratin, alpha 3,beta 1 integrin, and fibronectin. 
      
                        The cells are negative for factor VIII related antigen, 6.19 antigen and CALLA endopeptidase. 
      
                        HK-2 cells retain functional characteristics of proximal tubular epithelium such as Na+ dependent / phlorizin sensitive sugar transport and adenylate cyclase responsiveness to parathyroid, but not to antidiuretic hormone. 
      
                        The cells are capable of gluconeogenesis as evidenced by their ability to make and store glycogen. 
      
                        HK-2 cells are anchorage dependent. 
      
                        The cells will not grow in methylcellulose, soft agar or suspension. 
      
                        HK-2 cells can reproduce experimental results obtained with freshly isolated PTCs.      		
                    
      
                    
      
                        Propagation: 
                        ATCC complete growth medium: The base medium for this cell line is provided by Invitrogen (GIBCO) as part of a kit: Keratinocyte Serum Free Medium (K-SFM), Kit Catalog Number 17005-042. This kit is supplied with each of the two additives required to grow this cell line (bovine pituitary extract (BPE) and human recombinant epidermal growth factor (EGF). To make the complete growth medium, you will need to add the following components to the base medium:
                        
        
                            
      • 0.05 mg/ml BPE - provided with the K-SFM kit
        • 
                            
      • 5 ng/ml EGF - provided with the K-SFM kit. NOTE: Do not filter complete medium. 
        
                            Atmosphere: air, 95%; carbon dioxide (CO2), 5% 
        
                            Temperature: 37.0°C 
        
                            Growth Conditions: Cell growth is dependent on epidermal growth factor. The cells should not be allowed to become confluent. Subculture at 80% of confluence.
        • 
                        
      
                              		
                    
      
                    
      
                        Subculturing: 
                        Protocol: 
                        
        
                            
      1. Remove and discard culture medium.
        2. 
                            
      2. Briefly rinse the cell layer with 0.05% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
        3. 
                            
      3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
        
                            Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
        4. 
                            
      4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
        5. 
                            
      5. To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximately 125 xg for 5 to 10 minutes.Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.
        6. 
                            
      6. Incubate cultures at 37°C.
        7. 
                        
      
                              		
                    
      
                      
            
      
                  		
        
      
          

      
        
      
    
      

      
 
    
      

      

                            
      
    
      
    
      
        
      

  
      


          
      
              姓名：
              
      
                  
              
      
          
      
          
      
              電話：
              
      
                  
              
      
          
      
          
      
                  Email：
                  
      
                      
                  
      
              
      
          
      
              您的需求：
              
      
                  
              
      
          
      
          
      
               
              
      
                  
                  
              
      
          
      

  
      
  

      

    
      

      

                        
      
                    
      
                
      
            
      
        
      
    
      
                                            
      
                                        
      
                                    
      
                                
      
                            
      
                        
      
                    
      
                
      
                
            
      
		 		
      滬公網安備 31010902002429號
      
		 	
      
		 
      
        
      
    
      
 


主站蜘蛛池模板：
国产日韩欧美在线观看不卡|
日本免费成人网站|
无码2区|
97se亚洲综合自在线|
亚洲一区二区三区含羞草|
欧美精品人妻系列|
欧美性色19p|
av无码精品一区二区乱子|
18禁不禁短片|
草草浮力影院|
亚洲女同精品一区二区三区|
玖玖在线精品免费视频|
亚洲AV秘?无码一区二三月夜|
亚洲中文偷拍|
亚洲中文字幕2025|
色九九视频|
国产欧美日韩精品一区二区三区
|
成人国产片女人爽到高潮|
亚洲综合色婷婷中文字幕|
色综合av社区男人的天堂|
中文字幕日韩精品国产|
人人爽人人爽人人片a免费|
AV无码一区二区三区
|
日韩毛片在线免费观看|
国产精品中文久久久久久久
|
亚洲熟妇av午夜无码不卡|
阿片免费看|
www.啪啪白浆|
亚洲AV中文|
亚洲国产成人精品女人久久久|
人妻精品视频|
香蕉免费一区二区三区|
亚洲AV熟女|
国产人妻777人伦精品hd|
97超碰碰|
日日碰狠狠添天天爽超碰97|
国产久热精品热线av|
一本色道久久综合亚洲精品|
日韩影片一区二区三区|
久久香蕉国产线看观看猫咪av|
91一区二区在线播放精品|