產品詳情
簡單介紹：
CCL-97 R2C 大鼠睪丸間質細胞瘤，原代細胞|細胞系|細胞株|菌種；細胞庫管理規范，提供的細胞株背景清楚，提供參考文獻和Z優培養條件！
詳情介紹：
CCL-97 R2C 大鼠睪丸間質細胞瘤
ATCC® Number: CCL-97? Price: $438.00
Designations: R2C
Depositors: G Sato
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Rattus norvegicus (rat)
Morphology: epithelial
Source: Strain: WFu
Organ: testis
Disease: Leydig cell tumor
Cellular Products: steroid hormones; 20-hydroxypregn-4-en-3-one; progesterone
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host (Roche FuGENE® Transfection Reagents)
Virus Resistance: poliovirus 2
Reverse Transcript: positive
Age: 2 months
Gender: male
Comments: Stimulation by other hormones or cAMP is not required for hormone secretion.
Propagation: ATCC complete growth medium: The base medium for this cell line is F-12 Nutrient Mixture (Ham). To make the complete growth medium, add the following components to the base medium:
?fetal bovine serum to a final concentration of 2.5%
?horse serum to a final concentration of 15%
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Add fresh medium at least twice per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: recommended serum:ATCC 30-2020
recommended serum:ATCC 30-2040
References: 1100: Shin SI, et al. Studies on interstitial cells in tissue culture. II. Steroid biosynthesis by a clonal line of rat testicular interstitial cells. Endocrinology 82: 614-616, 1968. PubMed: 4868022
| ATCC® Number: | CCL-97? | Price: | $438.00 | |||||||||||||||||||||||||||||||||||||||||||||||||
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Designations: R2C
Depositors: G Sato
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Rattus norvegicus (rat)
Morphology: epithelial
Source: Strain: WFu
Organ: testis
Disease: Leydig cell tumor
Cellular Products: steroid hormones; 20-hydroxypregn-4-en-3-one; progesterone
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host (Roche FuGENE® Transfection Reagents)
Virus Resistance: poliovirus 2
Reverse Transcript: positive
Age: 2 months
Gender: male
Comments: Stimulation by other hormones or cAMP is not required for hormone secretion.
Propagation: ATCC complete growth medium: The base medium for this cell line is F-12 Nutrient Mixture (Ham). To make the complete growth medium, add the following components to the base medium:
?fetal bovine serum to a final concentration of 2.5%
?horse serum to a final concentration of 15%
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Add fresh medium at least twice per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: recommended serum:ATCC 30-2020
recommended serum:ATCC 30-2040
References: 1100: Shin SI, et al. Studies on interstitial cells in tissue culture. II. Steroid biosynthesis by a clonal line of rat testicular interstitial cells. Endocrinology 82: 614-616, 1968. PubMed: 4868022
