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      • 產品名稱：CTLL-2  小鼠T細胞 IL-2依賴型
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      • 產品型號：TIB-214
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      • 產品廠商：美國標準生物品收藏中心（ATCC）
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      • 產品價格：0
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            CTLL-2  小鼠T細胞IL-2依賴型，原代細胞|細胞系|細胞株|菌種；細胞庫管理規范，提供的細胞株背景清楚，提供參考文獻和培養條件！
        
      
    
      

    
      
        
      
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            CTLL-2  小鼠T細胞IL-2依賴型
      

      
	
		
      
			
				ATCC® Number: 
			
			
				TIB-214?    
			
			
				Price: 
			
			
				$289.00
			
		
      
		
      
			
				
      
					
						
      
							
								Designations: 
							
							
								CTLL-2
							
						
      
						
      
							
								Depositors: 
							
							
								 S Gillis 
							
						
      
						
      
							
								Biosafety Level:
							
							
								1
							
						
      
						
      
							
								Shipped: 
							
							
								frozen 
							
						
      
						
      
							
								Medium & Serum: 
							
							
								See Propagation
							
						
      
						
      
							
								Growth Properties:
							
							
								suspension
							
						
      
						
      
							
								Organism:
							
							
								Mus musculus (mouse)
							
						
      
						
      
							
								Morphology:
							
							
								lymphoblast
      
CTLL-2 小鼠T細胞 IL-2依賴型
      
      
							      		
						
      
						
      
							
								Source:
							
							
								Cell Type: lymphocyte cytotoxic T lymphocyte; 
      
Strain: C57BL/6      
							      		
						
      
						
      
							
								Permits/Forms:
							
							
								In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
							
						
      
						
      
							
								 
							
						
      
						
      
							
								Receptors:
							
							
								interleukin 2 (IL-2), expressed
							
						
      
						
      
							
								Comments:
							
							
								This line is a clone of cytotoxic T cells derived from a C57BL/6 mouse. The cells are dependent upon IL-2 for growth, and can be used to assay for IL-2.Immediately after thawing (or after overgrowth of a culture) the culture will appear to have few or no viable cells. This is normal.Depending upon the source and potency of the IL-2, it may take up to three weeks before the cells are ready to subculture.T-STIM with Con A (rat IL-2 culture supplement from Becton Dickinson) may be used or the rat factor may be prepared as described on the product sheet. The freshly prepared rat factor will usually produce more rapid growth.Tested and found negative for ectromelia virus (mousepox).
							
						
      
						
      
							
								Propagation: 
							
							
								ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: additional 2 mM L-glutamine; additional 1mM sodium pyruvate; adjust to a final concentration of 10% fetal bovine serum and 10% T-STIM with Con A. T-STIM is available from Becton Dickinson. 
      
Atmosphere: air, 95%; carbon dioxide (CO2), 5% 
      
Temperature: 37.0°C      
							      		
						
      
					      
				
      
			      		
		
      
	      

      
        
      
    
      

      
 
    
      

      

                            
      
    
      
    
      
        
      

  
      


          
      
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