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      • 產品名稱： Capan-2 人胰腺癌細胞
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      • 產品型號：HTB-80
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      • 產品廠商：美國標準生物品收藏中心（ATCC）
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      • 產品價格：0
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            HTB-79 Capan-2 人胰腺癌細胞，原代細胞|細胞系|細胞株|菌種；細胞庫管理規范，提供的細胞株背景清楚，提供參考文獻和培養條件！
        
      
    
      

    
      
        
      
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            HTB-79 Capan-2 人胰腺癌細胞

      
	
		
      
			
				ATCC® Number:  
			
			
				HTB-80?     
			
			
				Price:  
			
			
				$269.00 
			
		
      
		
      
			
				
      
					
						
      
							
								Designations:  
							
							
								Capan-2 
							
						
      
						
      
							
								Depositors:  
							
							
								 J Fogh  
							
						
      
						
      
							
								Biosafety Level:
							
							
								1 
							
						
      
						
      
							
								Shipped:  
							
							
								frozen  
							
						
      
						
      
							
								Medium & Serum:  
							
							
								See Propagation
							
						
      
						
      
							
								Growth Properties: 
							
							
								adherent 
							
						
      
						
      
							
								Organism: 
							
							
								Homo sapiens (human) 
							
						
      
						
      
							
								Morphology: 
							
							
								epithelial
      

      
       
							      		
						
      
						
      
							
								Source: 
							
							
								Organ: pancreas 
      
Disease: adenocarcinoma 
      
Derived from metastatic site: liver       
							      		
						
      
						
      
							
								Cellular Products: 
							
							
								mucin 
							
						
      
						
      
							
								Permits/Forms: 
							
							
								In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. 
							
						
      
					      
				
      
			      		
		
      
	      

      

      

      
	
		
      
			
				Comments: 
			
			
				The cells will slough off of the growth surface if they become too heavy. 
      
Capan-1 expresses the cystic fibrosis transmembrane conductance regulator (CFTR) and secrete gastric type mucins.       
			      		
		
      
		
      
			
				Propagation:  
			
			
				ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
      
Temperature: 37.0°C       
			      		
		
      
		
      
			
				Subculturing:  
			
			
				Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended 
      
Medium Renewal: Every 2 to 3 days 
      
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. 
      
Add fresh culture medium, aspirate and dispense into new culture flasks.       
			      		
		
      
		
      
			
				Preservation:  
			
			
				culture medium 95%; DMSO, 5% 
			
		
      
	      

      
        
      
    
      

      
 
    
      

      

                            
      
    
      
    
      
        
      

  
      


          
      
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