<cite id="pwzsq"><rp id="pwzsq"></rp></cite>


      
欧美视频精品免费覌看,7777久久亚洲中文字幕蜜桃,免费观看的av在线播放,国产一区二区三区免费主播,久久精品aⅴ无码中文字幕,中文字幕日韩有码国产,啊灬啊灬啊灬快灬深学长慢视频,国产片av片永久免费观看 

    
      
        
      
            
      
                
      
                    
                
      
                
                
      
                    
      
                        
      
                            
      
                                
      
                                    
                                
      
                                
      
                                    
                                    
      
                                        
      
                                            
      
                                                
      
        
      
        
      
            
      
                
      
                    
                    
                    
                    
                    
                
      
                
      
                
      
                    
      
                        
      
                            產品詳情
                        
      
                        
      
                            
      
                                
                                
                                
                                
                            
      
                            
      
    
      
    
      
        
      
    
      
            
      

                
      
                    
                    
        
                          
      • 產品名稱：B16F10 小鼠黑色素瘤高轉移細胞
        • 
                          
      • 產品型號：CRL-6475
        • 
                          
      • 產品廠商：美國標準生物品收藏中心（ATCC）
        • 
                          
      • 產品價格：0
        • 
                          
      • 折扣價格：0
        • 
                          
      • 產品文檔：
                          
        • 




                    
      
                    
        
                           
      • 
                             
        
                               
                             
        
                         
        • 
                           
      • 
                             
        
                               
                             
        
                         
        • 
                           
      • 
                             
        
                               
                             
        
                         
        • 
                    
      
                     
      
                    你添加了1件商品 查看購物車
                    
      

                
      
            
      
    
      

    
      
        
      
            簡單介紹：
        
      
        
      
            CRL-6475 B16F10 小鼠黑色素瘤高轉移細胞,原代細胞|細胞系|細胞株|菌種;細胞庫管理規范，提供的細胞株背景清楚，提供參考文獻和培養條件!
        
      
    
      

    
      
        
      
            詳情介紹：
        
      
        
      
            CRL-6475 B16F10 小鼠黑色素瘤高轉移細胞
      

      
	
		
      
			
				ATCC® Number:  
			
			
				CRL-6475?     
			
			
				Price:  
			
			
				$269.00 
			
		
      
		
      
			
				
      
					
						
      
							
								Designations:  
							
							
								B16-F10 
							
						
      
						
      
							
								Biosafety Level:
							
							
								1 
							
						
      
						
      
							
								Shipped:  
							
							
								frozen  
							
						
      
						
      
							
								Medium & Serum:  
							
							
								See Propagation
							
						
      
						
      
							
								Growth Properties: 
							
							
								adherent 
							
						
      
						
      
							
								Organism: 
							
							
								Mus musculus (mouse) 
							
						
      
						
      
							
								Morphology: 
							
							
								melanocyte
      
CRL-6475 B16F10 小鼠黑色素瘤高轉移細胞
      
       
							      		
						
      
						
      
							
								Source: 
							
							
								Organ: skin 
      
Strain: C57BL/6J 
      
Disease: melanoma       
							      		
						
      
						
      
							
								Permits/Forms: 
							
							
								In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. 
							
						
      
						
      
							
								 
							
						
      
						
      
							
								Applications: 
							
							
								transfection host (technology from amaxa) 
							
						
      
						
      
							
								Propagation:  
							
							
								ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
      
Temperature: 37.0°C 
      
Atmosphere: air, 95%; carbon dioxide (CO2), 5%       
							      		
						
      
						
      
							
								Subculturing:  
							
							
								Protocol:  
								
        
									
      1. 
										Remove and discard culture medium.
									
        2. 
									
      2. 
										Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
									
        3. 
									
      3. 
										Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
        
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
									
        4. 
									
      4. 
										Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
									
        5. 
									
      5. 
										Add appropriate aliquots of the cell suspension to new culture vessels.
									
        6. 
									
      6. 
										Incubate cultures at 37°C.
										
          
											
          
Subcultivation Ratio: A subcultivation ratio of 1:10 is recommended 
          
Medium Renewal: Every 2 to 3 days
										
        
									
        7. 
								
      
       
							      		
						
      
						
      
							
								Preservation:  
							
							
								Freeze medium: culture medium, 95%; DMSO, 5% 
      
Storage temperature: liquid nitrogen vapor phase       
							      		
						
      
					      
				
      
			      		
		
      
	      

      
        
      
    
      

      
 
    
      

      

                            
      
    
      
    
      
        
      

  
      


          
      
              姓名：
              
      
                  
              
      
          
      
          
      
              電話：
              
      
                  
              
      
          
      
          
      
                  Email：
                  
      
                      
                  
      
              
      
          
      
              您的需求：
              
      
                  
              
      
          
      
          
      
               
              
      
                  
                  
              
      
          
      

  
      
  

      

    
      

      

                        
      
                    
      
                
      
            
      
        
      
    
      
                                            
      
                                        
      
                                    
      
                                
      
                            
      
                        
      
                    
      
                
      
                
            
      
		 		
      滬公網安備 31010902002429號
      
		 	
      
		 
      
        
      
    
      
 


主站蜘蛛池模板：
婷婷伊人綜合中文字幕小说|
亚洲夜色噜噜av在线观看|
毛片免费试看|
国产乱子夫妻xx黑人xyx真爽|
1024国产视频|
国产人久久人人人人爽|
九九视频精品13在线观看|
国产一区二区三区不卡视频|
中文字幕av久久爽Av|
亚洲日韩中文字幕在线播放|
久久熟妇|
色噜噜狠狠色综合日日|
jizz国产|
又黄又爽又色又刺激的视频|
五月丁香六月激情综合|
久久青草国产精品一区|
亚洲AV一卡二卡|
亚洲精品9999久久久久无码
|
广昌县|
亚洲熟妇无码av另类vr影视|
久草国产视频|
久久久高清日本道免费观看|
91成人精品免费在线|
久久久久性色AV毛片特级|
性饥渴熟妇乱子伦HD|
91丨日韩丨精品丨乱码|
亚洲AV永久中文无码精品综合|
欧美阿V|
人禽交300部|
狠狠干奇米|
97人伦色伦成人免费视频|
亚洲国产精品羞羞|
成在线人免费无码高潮喷水|
国产三级va|
亚洲加勒比久久88色综合|
污污网站不卡|
亚洲性一交一乱一伦视频|
日韩特黄无码A片免费视频
|
久久国产一区二区日韩av|
日韩精品人妻系列无码专区|
人妻a码精品|