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      產品詳情
      • 產品名稱:AE-2 小鼠雜交瘤細胞抗AChE

      • 產品型號:HB-73
      • 產品廠商:美國標準生物品收藏中心(ATCC)
      • 產品價格:0
      • 折扣價格:0
      • 產品文檔:
      你添加了1件商品 查看購物車
      簡單介紹:
      AE-2 小鼠雜交瘤細胞抗AChE,原代細胞|細胞系|細胞株|菌種;細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻和培養條件!
      詳情介紹:
      AE-2  小鼠雜交瘤細胞抗AChE
      ATCC® Number: HB-73?    Price: $399.00
      Designations: AE-2
      Depositors:  DM Fambrough
      Isotype: IgG1
      Biosafety Level: 1
      Shipped: frozen
      Medium & Serum: See Propagation
      Growth Properties: suspension
      Organism: Mus musculus (B cell); Mus musculus (myeloma) (mouse (B cell); mouse (myeloma))
      Morphology: lymphoblast

      Source: Cell Type: hybridoma: B lymphocyte;
      Cellular Products: immunoglobulin; monoclonal antibody; against human acetylcholinesterase
      Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
       
      Comments: Animals were immunized with purified human erythrocyte acetylcholinesterase.
      Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
      The antibody cross-reacts with human and monkey neuromuscular junctions.
      Tested and found negative for ectromelia virus (mousepox).
      Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
      Subculturing: Medium Renewal: Every 2 to 3 days
      Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
      References: 939: Fambrough DM, et al. Acetylcholinesterase of human erythrocytes and neuromuscular junctions: homologies
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