CRL-8024 PLC/PRF/5 人肝癌亞力山大細胞
ATCC® Number: CRL-8024?
Designations: PLC/PRF/5
Depositors: WJ McAleer
Biosafety Level: 2 [Cells Contain HEPATITIS B ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: epithelial
Source: Organ: liver
Disease: hepatoma
Cell Type: Alexander cells;
Cellular Products: hepatitis virus B surface antigen (HBsAg)
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DNA Profile (STR): Amelogenin: X
CSF1PO: 10
D13S317: 11,12
D16S539: 13
D5S818: 12
D7S820: 9,11
THO1: 8
TPOX: 8
vWA: 15,16
Comments: The line was originally contaminated with mycoplasma, and was cured by treatment with BM-cycline.
The cells secrete HBsAg.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 22189: Monjardino J, Crawford E. Polypeptide profile of HBSAg excreted by a human hepatoma cell line. Virology 96: 652-655, 1979. PubMed: 223321
22198: MacNab GM, et al. Hepatitis B surface antigen produced by a human hepatoma cell line. Br. J. Cancer 34: 509-515, 1976. PubMed: 187208
22236: Skelly J, et al. Hepatitis B surface antigen produced by a human hepatoma cell line. Nature 282: 617-618, 1979. PubMed: 233138
22244: . . Science 209: 407, 1980.
22265: . . S. Afr. J. Med. Sci. 41: 8-9, 1976.
23123: Gilligan A, et al. Variation in EGF-induced EGF receptor downregulation in human hepatoma- derived cell lines expressing different amounts of EGF receptor. Exp. Cell Res. 200: 235-241, 1992. PubMed: 1315281
23525: Alexander JJ, et al. Establishment of a continuously growing cell line from primary carcinoma of the liver. S. Afr. Med. J. 50: 2124-2128, 1976. PubMed: 63998
