CRL-1825 P19 小鼠畸胎瘤細(xì)胞
ATCC® Number: CRL-1825?
Designations: P19
Depositors: MW McBurney
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus (mouse)
Morphology: epithelial
Source: Organ: embryo
Strain: C3H/He
Disease: teratocarcinoma; embryonal carcinoma
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Applications: transfection host (Nucleofection technology from Lonza)
Cytogenetic Analysis: n = 40; XY, n = 40; XY [22702]
Gender: male
Comments: The P19 line was derived from an embryonal carcinoma induced in a C3H/He mouse. [22702]
The line can be cloned at high efficiency in medium containing 0.1 mM 2-mercaptoethanol. [22702]
The cells are pluripotential.
The cell can be induced to differentiate into neural and glial like cells in the presence of 500 nM retinoic acid. [22492]
In the presence of 0.5% to 1.0% dimethylsulfoxide (DMSO) the cells differentiate to form cardiac and skeletal muscle-like elements, but do not form neural or glial like cells. [22913]
In the presence of both DMSO and retinoic acid, the cells differentiate as in the presence of retinoic acid alone. [22913]
Propagation: ATCC complete growth medium: The base medium for this cell line is Alpha Minimum Essential Medium with ribonucleosides and deoxyribonucleosides. To make the complete growth medium, add the following components to the base medium: bovine calf serum to a final concentration of 7.5%; fetal bovine serum to a final concentration of 2.5%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol: Do not allow the cells to become confluent.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:10 every 2 to 3 days is recommended
Medium Renewal: Add fresh medium at least every 48 hours
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: recommended serum:ATCC 30-2020
recommended serum:ATCC 30-2030
References: 21632: Bennicelli JL, et al. Mechanism for transcriptional gain of function resulting from chromosomal translocation in alveolar rhabdomyosarcoma. Proc. Natl. Acad. Sci. USA 93: 5455-5459, 1996. PubMed: 8643596
22492: Jones-Villeneuve EM, et al. Retinoic acid induces embryonal carcinoma cells to differentiate into neurons and glial cells. J. Cell Biol. 94: 253-262, 1982. PubMed: 7107698
22702: McBurney MW, Rogers BJ. Isolation of male embryonal carcinoma cells and their chromosome replication patterns. Dev. Biol. 89: 503-508, 1982. PubMed: 7056443
22913: McBurney MW, et al. Control of muscle and neuronal differentiation in a cultured embryonal carcinoma cell line. Nature 299: 165-167, 1982. PubMed: 7110336
