CCL-246 KG-1 人白血病細胞
ATCC® Number: CCL-246?
Designations: KG-1
Depositors: DW Golde
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens (human)
Morphology: myeloblast
Source: Organ: bone marrow
Disease: acute myelogenous leukemia
Cellular Products: HLA DR
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Applications: transfection host (technology from amaxa)
Reverse Transcript: negative
Antigen Expression: HLA A30, A31, B35, Cw4
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 7
D13S317: 11,12
D16S539: 10,11
D5S818: 13
D7S820: 8,10
THO1: 7,8
TPOX: 7,9
vWA: 14,19
Cytogenetic Analysis: The stemline chromosome number is near-diploid, with the 2S component occurring at 1.8%. Five markers (constitutive markers) were common to most, if not all, metaphases analyzed. Modal chromosome number is 47 (or 46 plus a small metacentric chromosome which is smaller than the G1 group chromosome). Normal chromosomes 5, 7, 8, 12 and 17 were monosomic, and others were disomic. The Y chromosome is detected in the Q-banded preparations.
Isoenzymes: AK-1, 0
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 1
PGM1, 1-2
PGM3, 0
Age: 59 years
Gender: male
Ethnicity: Caucasian
Comments: The KG-1 cell line was derived by H.P. Koeffler and D.W. Golde. A bone marrow aspirate was obtained from a 59-year-old Caucasian male with erythroleukemia that evolved into acute myelogenous leukemia. [1056]
KG-1 cells spontaneously differentiate to granulocyte and macrophage like cells.
They show a good response to colony stimulating factor (CSF). [1056]
The line is EBNA negative (EBNA-).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 (5) viable cells/ml. Maintain cell density between 2 X 10(5) and 1 X 10(6) viable cells/ml. Do not allow to exceed 2 X 10(6) cells/ml.
Medium Renewal: Twice per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
recommended serum:ATCC 30-2020
References: 867: Koeffler HP, Golde DW. Human myeloid leukemia cell lines: a review. Blood 56: 344-350, 1980. PubMed: 6996765
1056: Koeffler HP, Golde DW. Acute myelogenous leukemia: a human cell line responsive to colony-stimulating activity. Science 200: 1153-1154, 1978. PubMed: 306682
26069: . . Blood 54: 174a, 1979.
33055: Penrose JF, et al. Molecular cloning of the gene for human leukotriene C4 synthase. J. Biol. Chem. 271: 11356-11361, 1996. PubMed: 8626689
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